Table 2.
Plasmids and yeast strains
| Name | Genotype | Origin |
|---|---|---|
| Yeast strains | ||
| MW409 | MATa ade2-1 lys2-801 ura3-52 trp1- Δ63 his3- Δ200 rpc160- Δ1::HIS3 / pC160-6 (URA3 CEN4 RPC160) | (46) |
| SRY31 | MATα ura3-52 his3- Δ200 trp1- Δ1 lys2-801 ade2-1 ret1 Δ::HIS3 / Yep24-RET1 (2μ URA3 RET1) | (44) |
| SC116 | MATα rpc82::HIS3-1 ura3-52 trp1- Δ1 his3-Δ200 ade2-1 lys2-801 leu2-Δ / pRS314 (CEN4 TRP1 rpc82-6) | (C. Mann) |
| SC116-WT | MATα rpc82::HIS3-1 ura3-52 trp1- Δ1 his3-Δ200 ade2-1 lys2-801 leu2-Δ / Scp80 (URA3 CEN4 ARS1 RPC82) | This work |
| CMY356 | MATa rpc53::HIS3-1 ura3-52 trp1- Δ1 his3- Δ200 ade2-1 lys2-801 / pEMBLYc32-RPC53 (URA3 CEN4 ARS1 RPC53) | (C. Mann) |
| YMR2 | MATa rpc37::KanMX4 ade2-1 ura3-52 trp1-Δ63 his3-Δ200 leu2-Δ1 lys2-801 / pRS316-RPC37 (CEN4 URA3 RPC37) | (47) |
| D57-9A | MATa ade2-1 lys2-801 ura3-52 his3- Δ200 trp1- Δ1 rpc34-Δ::HIS3 / pYS34 (URA3 CEN4 RPC34) | (42,53) |
| CD8326 | MATa ade2-1 ura3-53 lys2-801 his3 trp1- Δ800 leu2-3rpc31- Δ::LEU2 / pC326 (2 μ URA3 RPC31) | (43) |
| FYBL3-2B | MATα ura3- Δ851 his3- Δ200 leu2- Δ1 trp1- Δ63 rpc25- Δ::KANMX4 / pFL44-RPC25 (2 μ URA3 RPC25) | (48) |
| YGP-25 | MATα ura3- Δ851 his3- Δ200 leu2- Δ1 trp1- Δ63 rpc25- Δ::KANMX4 / pGP-25 (2μ TRP1 rpc25+ Sch. pombe) | This work |
| YOL14 | MATa ade2 ura3 trp1 leu2 lys2 his3 rpc17::HIS3 / pRS316-RPC17 (URA3 CEN4 RPC17) | (49) |
| YGP-17 | MATa ade2 ura3 trp1 leu2 lys2 his3 rpc17::HIS3 / pGP-17 (2μ TRP1 rpc17+ Sch. pombe) | This work |