Figure 1.

Principle of RecA-mediated exon profiling method. Oligonucleotides were designed for exon candidate regions. (a) Incubation of the labeled oligonucletides (probes) with RecA, ATPγS and Mg²⁺ for 5 min at 37°C. (b) Incubation for 1 h at 37°C after adding dsDNA (e.g. pUC19) to the reaction mixture. (c) Electrophoresis and autoradiography for detection. The shifted band with slower migration represents triplex formation, indicating the presence of the sequence corresponding to the probe (arrow I). The bands for the probes without forming the triplex are represented as faster migration (arrow II).