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. 2006 Aug 8;34(13):e97. doi: 10.1093/nar/gkl497

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Examination of probe length with RecA-mediated exon profiling method. Two sets of 50, 40, 30, 20 and 10mer probes were prepared; identical (P) and non-identical (N) to pUC19 DNA. Identical (P) probes with 50, 40, 30, 20 and 10mer probes were examined in lanes 1, 3, 5, 7 and 9, respectively. Non-identical (N) probes with 50, 40, 30, 20 and 10mer were examined in lanes 2, 4, 6, 8 and 10, respectively. In lane 11, the reaction without RecA protein was examined. Open box indicates the position of the band corresponding to triplex. The DNA size marker (λHind III digests) is shown at the left with the approximate molecular sizes in kb. The sequences for all the probes are listed in Supplementary Table S1.