TABLE 2.
Expression of the paeX::uidA fusion
| Strain (main genotype) | Carbon source(s)a | Sp actb
|
|
|---|---|---|---|
| Pectate lyase | β-Glucuron- idase | ||
| A3785 (paeX::uidA) | Glycerol | 0.06 | 56 |
| Glycerol + PGAc | 3.26 | 207 | |
| Glucose | 0.04 | 29 | |
| Glucose + PGA | 1.25 | 109 | |
| A3910 (kdgK paeX::uidA) | Glycerol | 0.04 | 55 |
| Glycerol + PGA | 51.95 | 1,247 | |
| A3911 (kdgR paeX::uidA) | Glycerol | 0.63 | 327 |
| Glycerol + PGA | 2.81 | 646 | |
| A3912 (pecS paeX::uidA) | Glycerol | 0.66 | 110 |
| Glycerol + PGA | 8.70 | 389 | |
| A3913 (pecT paeX::uidA) | Glycerol | 0.97 | 52 |
| Glycerol + PGA | 8.01 | 184 | |
| A3914 (crp paeX::uidA) | Glucose | 0.01 | 25 |
| Glucose + PGA | 0.02 | 28 | |
a
Cultures were grown in M63 minimal medium to late log phase, in the absence or presence of polygalacturonate.
b
β-Glucuronidase specific activity reflects the expression of the paeX::uidA fusion and is expressed as nanomoles of product liberated per minute per milligram (dry weight) of bacteria. Pectate lyase specific activity is expressed as micromoles of product liberated per minute per milligram (dry weight) of bacteria. The results reported are the averages of at least three independent experiments, and the standard deviation was, in each case, less than 10% for β-glucuronidase and less than 20% for pectate lyase assays.
c
PGA, polygalacturonate.