Table 1.

Binding constants (μM) for 1xMBD and 4xMBD with probes that have 1, 2 or 3 methyl-CpG moieties (1ME, 2ME and 3ME)

Protein	1ME	2ME	3ME
4xMBD	0.5 µMa	0.05 µMa	0.02 µMa
1xMBD	30 µMa	3 µMa	2 µMa
4x/1x ratio	55	59	81

^aBinding constants were deduced from bandshift data shown in Figure 2 in which probe (25 fmol) was incubated in 100 mM salt with 3–300 nmol 1xMBD or 1–100 nmol 3xMBD (see Materials and Methods).