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. 2006 Aug 7;34(13):e98. doi: 10.1093/nar/gkl547

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sensitivity and yield of cHDA. (A) The sensitivity to the template amount of cHDA was tested. The amount of the pREP plasmid DNA (6 kb) used in each cHDA reaction is indicated on the top. Lane M, 2-log DNA maker (NEB). (B) Time course of cHDA product formation. A series of cHDA reactions containing different amounts of the pREP plasmid template (6 kb; 50, 5, 0.5 or 0.1 ng) was incubated at 25°C for upto 24 h and the incubation was stopped at various time points. The product formation was visualized by agarose gel electrophoresis, and analyzed with a GelDoc system (Bio-Rad). The relative yield (%) of the 2.3 kb specific product was calculated.