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. 2006 Aug 7;34(13):e98. doi: 10.1093/nar/gkl547

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Primer specificity of cHDA. Various primers were used in the cHDA reactions using pREP (6 kb) as a template. Each cHDA reaction contained one forward primer selected from S1201 (lanes 1), S1226 (lanes 2), S1233 (lanes 3) and S1249 (lanes 4) and one reverse primer chosen from S1224 (lanes a), S1248 (lanes b) and S1212 (lanes c). All of the primers carry sequences complementary to the pREP seqnence except two forward primers S1226 and S1249, and amplify the same region. The size of specific products is similar (2.3 kb). Lane M, 2-log DNA marker (NEB).