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. 2006 Aug 7;34(13):e98. doi: 10.1093/nar/gkl547

Figure 9.

Figure 9

Template preference of cHDA. Two different forms of circular DNA, cccDNA or nicked cDNA, were compared as a template in the cHDA reactions. Lane M, 2-log DNA marker (NEB). The pCR-4E plasmid DNA (4.5 kb) in the form of cccDNA (A: lane a) was converted to a nicked cDNA form by BbvCIB digestion (A: lane b). The one-step (B) or two-step (C) cHDA reactions were performed using PCR-4E as a template and S1233 and S1224 as primers: lanes a, cccDNA: lanes b, nicked DNA. The amount of pCR-4E used was 10 ng (lanes 1), 2.5 ng (lanes 2), 0.6 ng (lanes 3) or 0.16 ng (lanes 4).