FIG. 2.
Effect of substrate concentration on AdoMet transport in rickettsiae. KT values were obtained by nonlinear regression analysis with GraphPad Prism software. (A) Transport of AdoMet by R. prowazekii Madrid E at 34°C (▪), at 4°C (○), and in the presence of DNP (•). Nonspecific background binding of labeled AdoMet to filters is also shown (∗) and is subtracted from experimental values in panels B to D. (B) A composite of four independent R. prowazekii Madrid E AdoMet transport assays normalized to a Vmax of 100 pmol min−1 mg−1. (C) A composite of two independent R. prowazekii Breinl AdoMet transport assays normalized to a Vmax of 100 pmol min−1 mg−1. (D) A composite of two independent R. typhi Wilmington AdoMet transport assays normalized to a Vmax of 100 pmol min−1 mg−1.