FIG. 6.
Effect of various periplasmic proteases on pac overexpression. Same as Fig. 2 except the solid symbols represent cultures further supplemented with 0.05 g of arabinose per liter for induction of protease synthesis when the first sample was taken. Immunological analysis of the protein contents of the insoluble fractions of the last samples of various cultures is shown in panel D. Lanes: 1, pTrcKnPAC2902; 2, pTrcKnPAC2902 and pARDegP; 3, pTrcKnPAC2902 and pARDegPS210A; 4, pTrcKnPAC2902 and pARDegQ; 5, pTrcKnPAC2902 and pARDegS.