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Regulation of rocA expression by RocA is independent of functional CovR. RNA was extracted from the JRS4 (wild-type) and JRS948 (covR) strains at the late exponential (LE) and early stationary (ES) phases (arrows in panel A). Membranes were hybridized with rpsL, rocA, and sagA probes as described in Materials and Methods (B). The results shown are representative of hybridization from two independent RNA isolations.
