TABLE 1.

Relative distribution of ¹⁴C among the lipid classes derived from sodium [1 − ¹⁴C]propionic acid in M. tuberculosis and its msl7 and pks10 mutants

Classa	Relative distribution of 14C (%)
	Wild type	msl7 mutant	pks10 mutant
DIM	30	0	0
Fatty acids	28	0	0
Hydroxy fatty acids	2	0	0
PAT-I	16	18	20
Fatty acids	14	14	16
Mycolipanolic acids	1.7	3	3
Hydroxyphthioceranic acids	0.3	1	1
PAT-II	4	3	4
Fatty acids	1	1	1
Mycolipanolic acids	1	0.6	1
Hydroxyphthioceranic acids	2	1.4	2
Sulfolipids	45	73	70
Fatty acids	10	10	10
Mycolipanolic acids	4	10	9
Hydroxyphthioceranic acids	31	53	51
Very polar lipids	5	6	6
Fatty acids	0.5	1	1
Mycolipanolic acids	0.3	0.5	0.6
Hydroxyphthioceranic acids	4.2	4.5	4.4

^aPAT, polyacyl trehaloses; DIM, dimycocerosyl phthiocerols. Very polar lipids (including diacyl trehalose) remained at and near the origin on TLC with 10% methanol in chloroform as the solvent system.