TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant features | Source or reference |
|---|---|---|
| X. nematophila strains | ||
| HGB007 | ATCC 19061, wild type, Ampr | ATCC |
| HGB081 | Spontaneous Rifr mutant of X. nematophila ATCC 19061 | S. Forst |
| HGB340 | GFP+ derivative of HGB007, Cmlr | This study |
| HGB566 | Mini-Tn7-ECFP-containing derivative of HGB007, Gmr | This study |
| HGB567 | Mini-Tn7-DsRed gene-containing derivative of HGB007, Gmr | This study |
| STM-1 | HGB081 containing unique (probe A) signature-tagged mini-Tn5 | 13; this study |
| STM-2 | HGB081 containing unique (probe B) signature-tagged mini-Tn5 | 13; this study |
| STM-3 | HGB081 containing unique (probe C) signature-tagged mini-Tn5 | 13; this study |
| Plasmids | ||
| pNK2859 | Contains mini-Tn10-kan, source of PaphA | 29 |
| pQBI63 | Source of Super-Glo GFP gene | Qbiogene |
| pKR100 | oriR6K-based suicide vector, Cm1r | K. Visick |
| pKR100B | pKR100 with unique BgIII site | This study |
| pECM9 | pQBI63 with PaphA in place of PT7 | This study |
| pECM2 | PaphA-Super-Glo GFP gene fragment from pECM9 cloned into pKR100B | This study |
| pECM20 | pECM2 containing a 614-bp chromosomal insert from ATCC 19061 | This study |
| pBK-mini-Tn7(Gm)PA1/04/03-DsRed gene | Source of mini-Tn7-PA1/04/03-DsRed gene, Gmr | L. Lambertsen |
| pBK-mini-Tn7(Gm)PA1/04/03-ECFP gene | Source of mini-Tn7-PAl/04/03-ECFP gene, Gmr | L. Lambertsen |
| pUX-BF13 | Tn7 transposition helper plasmid (in E. coli S17-1 λ pir) | 5 |