TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| MG1655 | Prototrophic E. coli K-12 | CGSC 6300a |
| BE1815 | MG1655 crp::Sm | This study |
| IBPC 6411 | thi-1 argE3 his-4 proA2 lacY1 galK2 mtl-1 xyl-5 tsx-29 supE44 rpsL pps metZΔ2::cat | M. Springer |
| BE1723 | MG1655 metZΔ2::catZ | This study |
| Plasmids | ||
| pJCD01 | PUC19 derivative containing a polylinker flanked by the divergent terminators rpoCt and rrnBT1T2 | 24 |
| pKK232-8 | Cloning vector for promoter analysis | Pharmacia |
| pDIA530 | pKK232-8 derivative carrying the −417 to +90 argG promoter region | This study |
| pDIA539 | pJCD01 derivative carrying the −212 to +56 argG promoter region | This study |
| pDIA560 | As pDIA530 but with a mutated CAP site: AGTGAtccacgCCACA→ AGTAAtccacgCTCGAb | This study |
a
CGSC, E. coli Genetic Stock Center, Yale University.
b
Underlining indicates mutated residues.