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Summary of the purification of chlorate reductase from P. chloritidismutans
| Step | Vol (ml) | Protein concn (mg/ml) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|
| Soluble fraction | 13 | 27.5 | 726 | 2 | 100 | 1 |
| Q-Sepharose | 20 | 2.8 | 335 | 6 | 46.1 | 3 |
| Hydroxyapatite | 5 | 3.5 | 155 | 8.7 | 21.4 | 4.4 |
| Mono-Q | 2 | 3.3 | 95 | 14.4 | 13.1 | 7.2 |
| Superdex 200 | 10 | 0.18 | 75 | 42 | 10.3 | 21 |
