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Cross-linking of MBP-N-XylS*. E. coli bearing plasmids that will produce MBP-N-XylSL193A, MBP-N-XylSL194A, and MBP-N-XylSI205A were grown in the absence and in the presence of 3MBz. Proteins were purified as described in Materials and Methods, and samples were incubated in the presence (+) and in the absence (−) of 0.005% (vol/vol) glutaraldehyde.
