TABLE 1.
Strains and plasmids used in this study
| Strains or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 gyrA96 relA1 | 8 |
| JL1436 | deoC1 ptsF25 rbsR PsulA::′lacZ | 1 |
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 (Strr) relA1 flbB5301 | 18 |
| Plasmids | ||
| pCMX2 | pSELECT-1, xylS ori F1, ColE1, Tcr | 22 |
| pERD100 | pMP220, Pm:′lacZ, IncP1, Tcr | 33 |
| pGB002 | pSE380, LexA DNA binding domain (amino acids 1-87) | 1 |
| pLOW2 | pACYC177, p15A, Kmr | 9 |
| pLRRA1 | pLOW2 derivative bearing xylS mutant allele encoding XylSL193A | This study |
| pLRRA2 | As pLLAR1 but encoding XylSL194A | This study |
| pLRRA3 | As pLLAR1 but encoding XylSI205A | This study |
| pLRRA4 | As pLLAR1 but encoding XylSL193A, L194A | This study |
| pLRRA5 | As pLLAR1 but encoding XylS193A, 1205A | This study |
| pLRRA6 | As pLLAR1 but encoding XylSL194A, 1205A | This study |
| pLRRA7 | As pLLAR1 but encoding XylSL193A, L194A, I205A | This study |
| pLRRA8 | pGB002 derivative encoding chimeric N-XylS-LexA protein | This study |
| pLRRA9 | As pLRRA8 but encoding N-XylS L193A-LexA protein | This study |
| pLRRA10 | As pLRRA8 but encoding N-XylS L194A-LexA protein | This study |
| pLRRA11 | As pLRRA8 but encoding N-XylS I205A-LexA protein | This study |
| pLRRA12 | As pLRRA8 but encoding N-XylS L193A, L194A-LexA protein | This study |
| pLRRA13 | As pLRRA8 but encoding N-XylS L193A, I205A-LexA protein | This study |
| pLRRA14 | As pLRRA8 but encoding N-XylS L194A,I205A-LexA protein | This study |
| pLRRA15 | As pLRRA8 but encoding N-XylS L193A,L194A,I205A-LexA protein | This study |
| pMAL-pV | pMAL-C2, PtalmalE lacIq | 28, 29 |
a
Apr, Kmr, Smr, and Tcr stand for resistance to ampicillin, kanamycin, streptomycin, and tetracycline, respectively.