TABLE 3.
Induction of Pm by variants of XylS proteina
| N-XylS protein | β-Galactosidase (Miller units)
|
Induction ratio, +3MBz/−3MBz (fold increase) | |
|---|---|---|---|
| Without 3MBz | With 3MBz | ||
| Wild type | 50 ± 2 | 1,510 ± 80 | 30 |
| N-XylSL193A | 45 ± 1 | 450 ± 30 | 10 |
| N-XylSL194A | 50 ± 1 | 50 ± 5 | 1 |
| N-XylSI205A | 45 ± 3 | 1,650 ± 60 | 37 |
| N-XylSL193A,L194A | 45 ± 4 | 50 ± 8 | 1 |
| N-XylSL193A,I205A | 45 ± 2 | 60 ± 2 | 1 |
| N-XylSL194A,I205A | 45 ± 2 | 50 ± 1 | 1 |
| N-XylSL193A,L194A,I205A | 45 ± 2 | 45 ± 2 | 1 |
a
E. coli MC4100(pERD100) bearing a derivative of pLOW2 that encodes the indicated XylS protein was cultured as indicated in Materials and Methods. Data (values are rounded) are the averages of at least three independent assays performed in triplicate.