Table 2.

Secondary antibodies and probes used for immunofluorescence and western blot analysis.

Antibody/Probe (Catalog #)	Conjugate	Dilution	Supplier
Goat anti-mouse IgG (#A-11001)	Alexa-fluor 488	1:200 in PBS	Molecular Probes, Eugene, OR
Goat anti-rabbit IgG (#T-6778)	Alexa-fluor 488	1:200 in PBS	Molecular Probes, Eugene, OR
Goat anti-mouse polyvalent (#A-0162)	Alkaline Phosphatase	1:4000 in PBS	Sigma Chem. Co, St. Louis, MO
Goat anti-rabbit IgG (#A-3812)	Alkaline Phosphatase	1:30,000 in PBS	Sigma Chem. Co., St. Louis, MO
Nucleic Acid binding probe; TO-PRO-3 iodine (#T-3605)	---	1:1000 in PBS	Molecular Probes, Eugene, OR