Table 3.
Percent bulk cytolysis from splenocytes at various effector-to-target cell ratios induced by single immunizations with PLG-CTAB-p55 gag DNA, PLG-DDA-p55 gag DNA, and naked p55 gag DNA
| Formulation | DNA dose | Target/effect or cell ratio | % Cytolysis, Sv/B p7g |
|---|---|---|---|
| PLG/CTAB-p55 DNA | 1 μg | 60 | 71 |
| 15 | 55 | ||
| 4 | 31 | ||
| PLG/DDA-p55 DNA | 1 μg | 60 | 70 |
| 15 | 54 | ||
| 4 | 17 | ||
| DNA alone | 1 μg | 60 | 3 |
| 15 | 1 | ||
| 4 | 0 | ||
| Vaccinia-gag-pol | 2 × 107 pfu | 60 | 64 |
| 15 | 35 | ||
| 4 | 11 |
Vaccinia-gag-pol-injected mice served as a positive control in the assay. pfu, plaque-forming unit.