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Extracellular PKA expression is inversely related to hormone dependency of breast cancer cells. PKA activity was measured in cell extracts and conditioned medium of hormone-dependent (MCF-7, T-47D, MCF-7TH) and -independent (SK-BR-3, MDA-MB-231) human breast carcinoma cells by the method described in Experimental Procedures. Statistical analysis was performed by the method described in the Fig. 1 legend. Data represent mean values ± SD of three separate experiments.
