Figure 1.

Time course of IgG anti-goat response in CBA mice immunized with various antigens. All sera were diluted 300× for this ELISA assay. Four mice were immunized in each group, except for group 5, which included 3 mice. The antigen received by each group was as follows: Group 1, polyclonal goat anti-hamster IgG antibody; Group 2, mAb N418; Group 3, hamster anti-TNP antibody; Group 4, mixture of goat anti-hamster antibody and mAb N418; and Group 5, mixture of goat anti-hamster IgG antibody and hamster anti-TNP antibody. Each mouse was given 0.5 μg of each indicated protein on day 0. The data points shown for day 0 correspond to sera obtained before immunization. Postimmunization serum samples were collected on days 3 and 7, followed by weekly collections.