Abstract
Crude human decidual extracts containing up to 15.0 mg/l PP14 were investigated for their effects on the release of interleukin-2 (IL-2) and of IL-2 receptor (IL-2R) from phytohaemagglutinin (PHA) stimulated lymphocytes. The crude decidual extract suppressed IL-2 production over the culture period investigated (0-90 h) with maximal suppression observed at around 66 h of culture. The suppression was dose dependent over the range of PP14 concentrations used (0-7.0 mg/l). Decidual extracts also inhibited the release of soluble IL-2R into the culture supernatants over the same period. In both cases, the specific reduction of the PP14 content of the extracts by a monoclonal anti-PP14 immunoadsorbent reduced the observed suppression. These results suggest that PP14 inhibits the production of IL-2 from mitogenically stimulated lymphocytes, and leads to a reduced IL-2R release. The mechanism for such an effect is as yet unknown; however, it may explain the previously reported immunosuppressive activity of PP14.
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- Baran D., Moreau J. L., Thèze J. New radioimmunoassay for the detection of free IL2 receptor in supernatants of murine helper and cytotoxic T-cell lines. Ann Inst Pasteur Immunol. 1986 Jul-Aug;137D(1):23–31. [PubMed] [Google Scholar]
- Bolton A. E., Chapman M. G., Stoker R. J., Andrew C. E., Wass D., Bohn H. The radioimmunoassay of human placental protein 14 (PP14). Clin Chim Acta. 1983 Dec 30;135(3):283–291. doi: 10.1016/0009-8981(83)90287-5. [DOI] [PubMed] [Google Scholar]
- Bolton A. E., Pockley A. G., Clough K. J., Mowles E. A., Stoker R. J., Westwood O. M., Chapman M. G. Identification of placental protein 14 as an immunosuppressive factor in human reproduction. Lancet. 1987 Mar 14;1(8533):593–595. doi: 10.1016/s0140-6736(87)90235-2. [DOI] [PubMed] [Google Scholar]
- Depper J. M., Leonard W. J., Krönke M., Noguchi P. D., Cunningham R. E., Waldmann T. A., Greene W. C. Regulation of interleukin 2 receptor expression: effects of phorbol diester, phospholipase C, and reexposure to lectin or antigen. J Immunol. 1984 Dec;133(6):3054–3061. [PubMed] [Google Scholar]
- Domingo C. G., Domenech N., Aparicio P., Palomino P. Human pregnancy serum inhibits proliferation of T8-depleted cells and their interleukin-2 synthesis in mixed lymphocyte cultures. J Reprod Immunol. 1985 Nov;8(2-3):97–110. doi: 10.1016/0165-0378(85)90033-6. [DOI] [PubMed] [Google Scholar]
- Garcia Domingo C., Moreno A., Palomino P. The effect of human pregnancy serum on the synthesis and action of interleukin-1. J Reprod Immunol. 1988 Jun;13(1):17–30. doi: 10.1016/0165-0378(88)90045-9. [DOI] [PubMed] [Google Scholar]
- Julkunen M., Rutanen E. M., Koskimies A., Ranta T., Bohn H., Seppälä M. Distribution of placental protein 14 in tissues and body fluids during pregnancy. Br J Obstet Gynaecol. 1985 Nov;92(11):1145–1151. doi: 10.1111/j.1471-0528.1985.tb03027.x. [DOI] [PubMed] [Google Scholar]
- Nicholas N. S., Panayi G. S., Nouri A. M. Human pregnancy serum inhibits interleukin-2 production. Clin Exp Immunol. 1984 Dec;58(3):587–595. [PMC free article] [PubMed] [Google Scholar]
- Pockley A. G., Mowles E. A., Stoker R. J., Westwood O. M., Chapman M. G., Bolton A. E. Suppression of in vitro lymphocyte reactivity to phytohemagglutinin by placental protein 14. J Reprod Immunol. 1988 Jun;13(1):31–39. doi: 10.1016/0165-0378(88)90046-0. [DOI] [PubMed] [Google Scholar]
- Robb R. J., Greene W. C., Rusk C. M. Low and high affinity cellular receptors for interleukin 2. Implications for the level of Tac antigen. J Exp Med. 1984 Oct 1;160(4):1126–1146. doi: 10.1084/jem.160.4.1126. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubin L. A., Kurman C. C., Fritz M. E., Biddison W. E., Boutin B., Yarchoan R., Nelson D. L. Soluble interleukin 2 receptors are released from activated human lymphoid cells in vitro. J Immunol. 1985 Nov;135(5):3172–3177. [PubMed] [Google Scholar]