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. 1986 Apr;64(1):181–187.

Mitogenic response of neoplastic B cells: comparison of reactivity to Staphylococcus aureus Cowan I and anti-immunoglobulin antibodies.

T Tamaki, T Yonezawa, Y Kanakura, S Katagiri, H Mizutani, T Kitani, S Tarui
PMCID: PMC1542155  PMID: 3015456

Abstract

Neoplastic B cells from two patients with hyperleucocytic hairy cell leukaemia (HCL) and 19 patients with chronic lymphocytic leukaemia of B cell Type (B-CLL) were investigated to examine the mitogenic responses to the F(ab')2 fraction of anti-human immunoglobulins (anti-Igs) and Staphylococcus aureus Cowan I (STA). Neoplastic cells from both HCL patients lacked surface Tac antigen. Mononuclear cells from the two HCL patients strongly responded to both anti-Igs and STA as measured by 3H-thymidine incorporation in vitro. Although the mononuclear cells from two patients with B-CLL showed high response to STA, cells from none of the patients with B-CLL responded to anti-Igs. Mononuclear cells as well as T-cell-depleted fractions from the two HCL patients showed a strong proliferative response by anti-gamma chain antibody (anti-gamma) and the mononuclear cells from one of the patients were also induced to proliferate by anti-delta, whereas those from normal subjects responded only to a high concentration of anti-mu. Based on the difference in reactivity to anti-Ig, it is suggested that the HCL cells in this study originate from a subset equivalent to 'memory' B cells, whereas the B-CLL cells originate from a subset equivalent to 'virgin' B cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson K. C., Boyd A. W., Fisher D. C., Leslie D., Schlossman S. F., Nadler L. M. Hairy cell leukemia: a tumor of pre-plasma cells. Blood. 1985 Mar;65(3):620–629. [PubMed] [Google Scholar]
  2. Boyd A. W., Metcalf D. Activation of human and murine B cells by anti-immunoglobulin antibody: dependence of the response on the preexisting level of B-cell activation. Cell Immunol. 1984 Nov;89(1):75–83. doi: 10.1016/0008-8749(84)90199-0. [DOI] [PubMed] [Google Scholar]
  3. Cawley J. C., Burns G. F., Hayhoe F. G. A chronic lymphoproliferative disorder with distinctive features: a distinct variant of hairy-cell leukaemia. Leuk Res. 1980;4(6):547–559. doi: 10.1016/0145-2126(80)90066-1. [DOI] [PubMed] [Google Scholar]
  4. Chiorazzi N., Fu S. M., Kunkel H. G. Stimulation of human B lymphocytes by antibodies to IgM and IgG: functional evidence for the expression of IgG on B-lymphocyte surface membranes. Clin Immunol Immunopathol. 1980 Mar;15(3):301–313. doi: 10.1016/0090-1229(80)90042-2. [DOI] [PubMed] [Google Scholar]
  5. Falkoff R. J., Zhu L. P., Fauci A. S. Separate signals for human B cell proliferation and differentiation in response to Staphylococcus aureus: evidence for a two-signal model of B cell activation. J Immunol. 1982 Jul;129(1):97–102. [PubMed] [Google Scholar]
  6. Forsgren A., Svedjelund A., Wigzell H. Lymphocyte stimulation by protein A of Staphylococcus aureus. Eur J Immunol. 1976 Mar;6(3):207–213. doi: 10.1002/eji.1830060312. [DOI] [PubMed] [Google Scholar]
  7. Fu S. M., Chiorazzi N., Kunkel H. G. Differentiation capacity and other properties of the leukemic cells of chronic lymphocytic leukemia. Immunol Rev. 1979;48:23–44. doi: 10.1111/j.1600-065x.1979.tb00297.x. [DOI] [PubMed] [Google Scholar]
  8. Harada H., Kasahara T., Ogata K., Shioiri-Nakano K., Kawai T. Effect of Staphylococcus aureus Cowan i bacteria on the mitogenic response of human B-cell subsets. Immunology. 1982 Nov;47(3):557–567. [PMC free article] [PubMed] [Google Scholar]
  9. Jansen J., LeBien T. W., Kersey J. H. The phenotype of the neoplastic cells of hairy cell leukemia studied with monoclonal antibodies. Blood. 1982 Mar;59(3):609–614. [PubMed] [Google Scholar]
  10. Katayama I., Mochino T., Honma T., Fukuda M. Hairy cell leukemia: a comparative study of Japanese and non-Japanese patients. Semin Oncol. 1984 Dec;11(4 Suppl 2):486–492. [PubMed] [Google Scholar]
  11. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  12. Korsmeyer S. J., Greene W. C., Cossman J., Hsu S. M., Jensen J. P., Neckers L. M., Marshall S. L., Bakhshi A., Depper J. M., Leonard W. J. Rearrangement and expression of immunoglobulin genes and expression of Tac antigen in hairy cell leukemia. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4522–4526. doi: 10.1073/pnas.80.14.4522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kumagai K., Abo T., Sekizawa T., Sasaki M. Studies of surface immunoglobulins on human B lymphocytes. I. Dissociation of cell-bound immunoglobulins with acid pH or at 37 degrees C. J Immunol. 1975 Oct;115(4):982–987. [PubMed] [Google Scholar]
  14. Leonard W. J., Depper J. M., Uchiyama T., Smith K. A., Waldmann T. A., Greene W. C. A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor; partial characterization of the receptor. Nature. 1982 Nov 18;300(5889):267–269. doi: 10.1038/300267a0. [DOI] [PubMed] [Google Scholar]
  15. Mond J. J., Schaefer M., Smith J., Finkelman F. D. Lyb-5- B cells of CBA/N mice can be induced to synthesize DNA by culture with insolubilized but not soluble anti-Ig. J Immunol. 1983 Nov;131(5):2107–2109. [PubMed] [Google Scholar]
  16. Muraguchi A., Butler J. L., Kehrl J. H., Fauci A. S. Differential sensitivity of human B cell subsets to activation signals delivered by anti-mu antibody and proliferative signals delivered by a monoclonal B cell growth factor. J Exp Med. 1983 Feb 1;157(2):530–546. doi: 10.1084/jem.157.2.530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Posnett D. N., Chiorazzi N., Kunkel H. G. Monoclonal antibodies with specificity for hairy cell leukemia cells. J Clin Invest. 1982 Aug;70(2):254–261. doi: 10.1172/JCI110612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Romagnani S., Giudizi G. M., Almerigogna F., Biagiotti R., Bellesi G., Bernardi F., Ricci M. Protein A reactivity of IgM- and IgD-bearing lymphocytes from some patients with chronic lymphocytic leukemia. Clin Immunol Immunopathol. 1981 Apr;19(1):139–148. doi: 10.1016/0090-1229(81)90055-6. [DOI] [PubMed] [Google Scholar]
  19. Romagnani S., Giudizi G. M., Almerigogna F., Nicoletti P. L., Ricci M. Protein A of Staphylococcus aureus is mitogenic for IgG-bearing, but also for a subpopulation of IgM- and/or IgD-bearing human lymphocytes. Immunology. 1980 Mar;39(3):417–425. [PMC free article] [PubMed] [Google Scholar]
  20. SELL S., GELL P. G. STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM. J Exp Med. 1965 Aug 1;122:423–440. doi: 10.1084/jem.122.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sieckmann D. G., Habbersett R., Scher I., Paul W. E. Activation of mouse lymphocytes by anti-immunoglobulin. III. Analysis of responding B lymphocytes by flow cytometry and cell sorting. J Immunol. 1981 Jul;127(1):205–211. [PubMed] [Google Scholar]
  22. Tamaki T., Yonezawa T., Kanayama Y., Inoue R., Kanakura Y., Katagiri S., Machii T., Kitani T., Tarui S. Mitogenic response of neoplastic "hairy cells" to Staphylococcus aureus Cowan I. Nihon Ketsueki Gakkai Zasshi. 1985 Jul;48(4):1042–1052. [PubMed] [Google Scholar]
  23. Tsudo M., Uchiyama T., Uchino H. Expression of Tac antigen on activated normal human B cells. J Exp Med. 1984 Aug 1;160(2):612–617. doi: 10.1084/jem.160.2.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. West W. H., Payne S. M., Weese J. L., Herberman R. B. Human T lymphocyte subpopulations: correlation between E-rosette-forming affinity and expression of the Fc receptor. J Immunol. 1977 Aug;119(2):548–554. [PubMed] [Google Scholar]
  25. Yoshizaki K., Nakagawa T., Kaieda T., Muraguchi A., Yamamura Y., Kishimoto T. Induction of proliferation and Ig production in human B leukemic cells by anti-immunoglobulins and T cell factors. J Immunol. 1982 Mar;128(3):1296–1301. [PubMed] [Google Scholar]
  26. Zola H., McNamara P. J., Moore H. A., Smart I. J., Brooks D. A., Beckman I. G., Bradley J. Maturation of human B lymphocytes--studies with a panel of monoclonal antibodies against membrane antigens. Clin Exp Immunol. 1983 Jun;52(3):655–664. [PMC free article] [PubMed] [Google Scholar]

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