Figure 3.

Inhibition of CpG 5mer-induced collapse of Δψ_m in MOLT-4 cells by Ac-DEVD-CHO without increase in cell survival. (A) The percentage of cells exhibiting red fluorescence from the dye JC-1, typical of normal mitochondrial transmembrane potential, was determined by flow cytometry. The oligodeoxynucleotide CGTTA, inverse control ATTGC, and caspase 3 inhibitor were delivered into cells by streptolysin O permeabilization at 0 h all from an extracellular concentration of 20 µM. SLO only: cells were permeabilized with streptolysin O in serum-free medium alone. (B) Cell number per ml excluding propidium iodide was determined by flow cytometry. Plotted are the mean and standard deviation of two replicates.