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Sedimentation profiles of Domain 2 RNA and ribosomal subunits. 33P-labeled Domain 2 RNA was incubated with purified (A) 40S or (B) 60S ribosomes and centrifuged in sucrose gradients. Fractions were collected and the radioactivity in each fraction was measured. Black and white arrowheads indicate positions of 40S and 60S subunits, respectively, which were monitored by absorbance at 254 nm.
