Figure 6.

Filter binding analyses of complete IRES RNA (Domains 1–2–3) and truncated IRES RNA (Domains 1–2) binding to programmed 80S ribosomes. (A) Effects of poly(U) and/or tRNA^Phe programming on RNA binding. (B) Effects of poly(U) and/or 17mer tRNA^Phe ASL programming on RNA binding. ³²P-labeled IRES RNAs were incubated with 80S ribosomes that had been preincubated with poly(U), tRNA^Phe and/or ASL. Binding for samples containing 1 pmol RNA and 4 pmol ribosomes [without poly(U), tRNA^Phe or ASL] was taken as 100% binding. Results are the average of three independent experiments. Bars represent standard errors.