Table 1. Luciferase activity of PSIV IRES variants with mutations in the internal bulge of Domain 3.
Sequencea | Relative light unitsb | Relative luciferase activity | |
---|---|---|---|
Wild-type | CUCAA6153–6157 | 48 445 ± 9843 | 100% |
GUGU6175–6172 | |||
C6173 | CUCAA6153–6157 | 19 275 ± 1465 | 39.8% |
GUCU6175–6172 | |||
C6173 + G6155 | CUGAA6153–6157 | 51 923 ± 3964 | 107.2% |
GUCU6175–6172 |
aUnderlining indicates mutated nucleotides.
bStandard deviations are also indicated.