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Purification and partial sequencing of pig 3′ tRNase. (A) The enzyme was intensively fractionated from pig liver basically as described before (10), and further purified by glycerol gradient ultracentrifugation. The final active fraction was separated on an SDS–10% polyacrylamide gel. (B) The four sequences derived from mass spectrometric analysis of the ∼85 kDa band are aligned with a C-terminal portion of the human ELAC2 sequence.
