Abstract
A component of Aspergillus fumigatus, Ag 7, previously identified as a major antigen for patients with allergic bronchopulmonary aspergillosis, has been isolated by gel filtration and further purified by affinity chromatography using monospecific antiserum. The antigen, which binds both specific IgG and IgE antibodies, was shown to be a high molecular weight, 150-200 kD, heat-stable glycoprotein, which binds to concanavalin A, suggesting the presence of alpha-D mannopyrannoside, or alpha-D glucopyrannoside end residues. On sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) it had a subunit of molecular weight 36 kD (with or without prior reduction), which retained antigenicity and allergenicity when tested with patients' sera.
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- Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
- Calvanico N. J., Du Pont B. L., Huang C. J., Patterson R., Fink J. N., Kurup V. P. Antigens of aspergillus fumigatus. 1. Purification of a cytoplasmic antigen reactive with sera of patients with aspergillus-related disease. Clin Exp Immunol. 1981 Sep;45(3):662–671. [PMC free article] [PubMed] [Google Scholar]
- De Magaldi S. W., Mackenzie D. W. Specificity of antigens from pathogenic Aspergillus species. I. Studies with ELISA and immunofluorescence. Sabouraudia. 1984;22(5):381–394. [PubMed] [Google Scholar]
- Dessaint J. P., Bout D., Fruit J., Capron A. Serum concentration of specific IgE antibody against Aspergillus fumigatus and identification of the fungal allergen. Clin Immunol Immunopathol. 1976 May;5(3):314–319. doi: 10.1016/0090-1229(76)90039-8. [DOI] [PubMed] [Google Scholar]
- Hansen B. L., Vestergaard B. F., Hansen G. N. Production of monospecific antibodies to varicella zoster virus antigen in rabbits tolerant to human IgG and immunized with antigen immunoprecipitated with human IgG from zoster reconvalescent sera. J Immunol Methods. 1981;43(3):283–290. doi: 10.1016/0022-1759(81)90176-9. [DOI] [PubMed] [Google Scholar]
- Hornsleth A., Mordhorst C. H., Pedersen I. R. New method for production of influenza A virus subtype specific antisera: employment of precipitate obtained by crossed immunoelectrophoretic techniques as antigen. J Immunol Methods. 1980;39(1-2):95–100. doi: 10.1016/0022-1759(80)90298-7. [DOI] [PubMed] [Google Scholar]
- Kauffman H. F., de Vries K. Antibodies against Aspergillus fumigatus. I. Standardization of the antigenic composition. Int Arch Allergy Appl Immunol. 1980;62(3):252–264. [PubMed] [Google Scholar]
- Kurup V. P., Fink J. N., Scribner G. H., Falk M. J. Antigenic variability of Aspergillus fumigatus strains. Microbios. 1977;19(77-78):191–204. [PubMed] [Google Scholar]
- Longbottom J. L. Allergic bronchopulmonary aspergillosis: reactivity of IgE and IgG antibodies with antigenic components of Aspergillus fumigatus (IgE/IgG antigen complexes). J Allergy Clin Immunol. 1983 Dec;72(6):668–675. doi: 10.1016/0091-6749(83)90627-9. [DOI] [PubMed] [Google Scholar]
- Longbottom J. L. Immunological aspects of infection and allergy due to Aspergillus species. Mykosen Suppl. 1978;1:207–217. [PubMed] [Google Scholar]
- Piechura J. E., Huang C. J., Cohen S. H., Kidd J. M., Kurup V. P., Calvanico N. J. Antigens of Aspergillus fumigatus. II. Electrophoretic and clinical studies. Immunology. 1983 Aug;49(4):657–665. [PMC free article] [PubMed] [Google Scholar]
- Schønheyder H., Andersen P. Fractionation of Aspergillus fumigatus antigens by hydrophobic interaction chromatography and gel filtration. Int Arch Allergy Appl Immunol. 1984;73(3):231–236. doi: 10.1159/000233473. [DOI] [PubMed] [Google Scholar]
- Schønheyder H., Andersen P. IgG antibodies to purified Aspergillus fumigatus antigens determined by enzyme-linked immunosorbent assay. Int Arch Allergy Appl Immunol. 1984;74(3):262–269. doi: 10.1159/000233555. [DOI] [PubMed] [Google Scholar]
- Steinbuch M., Audran R. The isolation of IgG from mammalian sera with the aid of caprylic acid. Arch Biochem Biophys. 1969 Nov;134(2):279–284. doi: 10.1016/0003-9861(69)90285-9. [DOI] [PubMed] [Google Scholar]
- Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem. 1969 Jul;30(1):148–152. doi: 10.1016/0003-2697(69)90383-2. [DOI] [PubMed] [Google Scholar]