Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1986 Aug;65(2):223–231.

Detection and quantification of circulating antigen in schistosomiasis by a monoclonal antibody. I. Specificity analysis of a monoclonal antibody with immunodiagnostic capacity.

J A Nogueira-Queiroz, C Lutsch, M Capron, J P Dessaint, A Capron
PMCID: PMC1542302  PMID: 3098474

Abstract

Monoclonal antibodies were obtained after immunization of mice with Schistosoma mansoni excretory/secretory antigen, previously shown to contain the circulating cathodic (M) antigen. Among these, the 40:B1 monoclonal antibody proved to be specific for the schistosome genus and to detect only adult worm-derived antigens as shown both by immunoprecipitation and with a two-site immunoradiometric assay using the monoclonal as both the solid-phase and the labelled antibody. The two-site immunoradiometric assay allows a sensitive measurement (detection limit: 5 ng) of circulating schistosome antigen in blood and in urine from patients with schistosomiasis. The amount of circulating schistosome M antigen is correlated with schistosome egg excretion in stool.

Full text

PDF
226

Images in this article

227Fig. 2
on p.227

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdel-Hafez S. K., Phillips S. M., Zodda D. M. Schistosoma mansoni: detection and characterization of antigens and antigenemia by inhibition enzyme-linked immunosorbent assay (IELISA). Exp Parasitol. 1983 Apr;55(2):219–232. doi: 10.1016/0014-4894(83)90016-4. [DOI] [PubMed] [Google Scholar]
  2. Addison G. M., Hales C. N. Two site assay of human growth hormone. Horm Metab Res. 1971 Jan;3(1):59–60. doi: 10.1055/s-0028-1096761. [DOI] [PubMed] [Google Scholar]
  3. Andrade Z. A., Sadigursky M. Immunofluorescence studies of schistosome structures which share determinants with circulating schistosome antigens. Trans R Soc Trop Med Hyg. 1978;72(3):316–317. doi: 10.1016/0035-9203(78)90217-1. [DOI] [PubMed] [Google Scholar]
  4. Berggren W. L., Weller T. H. Immunoelectrophoretic demonstration of specific circulating antigen in animals infected with Schistosoma mansoni. Am J Trop Med Hyg. 1967 Sep;16(5):606–612. doi: 10.4269/ajtmh.1967.16.606. [DOI] [PubMed] [Google Scholar]
  5. Boros D. L., Warren K. S. Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs. J Exp Med. 1970 Sep 1;132(3):488–507. doi: 10.1084/jem.132.3.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Capron A., Biguet J., Vernes A., Afchain D. Structure antigénique des helminthes. Aspects immunologiques des relations hote-parasite. Pathol Biol. 1968 Feb;16(3):121–138. [PubMed] [Google Scholar]
  7. Carlier Y., Bout D., Bina J. C., Camus D., Figueiredo J. F., Capron A. Immunological studies in human schistosomiasis. I. Parasitic antigen in urine. Am J Trop Med Hyg. 1975 Nov;24(6 Pt 1):949–954. doi: 10.4269/ajtmh.1975.24.949. [DOI] [PubMed] [Google Scholar]
  8. Carlier Y., Bout D., Capron A. Further studies on the circulating M antigen in human and experimental Schistosoma mansoni infections. Ann Immunol (Paris) 1978 Oct-Dec;129 100(6):811–818. [PubMed] [Google Scholar]
  9. Carlier Y., Bout D., Strecker G., Debray H., Capron A. Purification, immunochemical, and biologic characterization of the Schistosoma circulating M antigen. J Immunol. 1980 May;124(5):2442–2450. [PubMed] [Google Scholar]
  10. Carlier Y., Nzeyimana H., Bout D., Capron A. Evaluation of circulating antigens by a sandwich radioimmunoassay, and of antibodies and immune complexes, in Schistosoma mansoni-infected African parturients and their newborn children. Am J Trop Med Hyg. 1980 Jan;29(1):74–81. doi: 10.4269/ajtmh.1980.29.74. [DOI] [PubMed] [Google Scholar]
  11. Deelder A. M., Klappe H. T., van den Aardweg G. J., van Meerbeke E. H. Schistosoma mansoni: demonstration of two circulating antigens in infected hamsters. Exp Parasitol. 1976 Oct;40(2):189–197. doi: 10.1016/0014-4894(76)90081-3. [DOI] [PubMed] [Google Scholar]
  12. Deelder A. M., Kornelis D., Van Marck E. A., Eveleigh P. C., Van Egmond J. G. Schistosoma mansoni: characterization of two circulating polysaccharide antigens and the immunological response to these antigens in mouse, hamster, and human infections. Exp Parasitol. 1980 Aug;50(1):16–32. doi: 10.1016/0014-4894(80)90004-1. [DOI] [PubMed] [Google Scholar]
  13. Dissous C., Prata A., Capron A. Human antibody response to Schistosoma mansoni surface antigens defined by protective monoclonal antibodies. J Infect Dis. 1984 Feb;149(2):227–233. doi: 10.1093/infdis/149.2.227. [DOI] [PubMed] [Google Scholar]
  14. Feldmeier H., Nogueira-Queiroz J. A., Peixoto-Queiroz M. A., Doehring E., Dessaint J. P., de Alencar J. E., Dafalla A. A., Capron A. Detection and quantification of circulating antigen in schistosomiasis by monoclonal antibody. II. The quantification of circulating antigens in human schistosomiasis mansoni and haematobium: relationship to intensity of infection and disease status. Clin Exp Immunol. 1986 Aug;65(2):232–243. [PMC free article] [PubMed] [Google Scholar]
  15. Gold R., Rosen F. S., Weller T. H. A specific circulating antigen in hamsters infected with Schistosoma mansoni. Detection of antigen in serum and urine, and correlation between antigenic concentration and worm burden. Am J Trop Med Hyg. 1969 Jul;18(4):545–552. doi: 10.4269/ajtmh.1969.18.545. [DOI] [PubMed] [Google Scholar]
  16. Grzych J. M., Capron M., Bazin H., Capron A. In vitro and in vivo effector function of rat IgG2a monoclonal anti-S. mansoni antibodies. J Immunol. 1982 Dec;129(6):2739–2743. [PubMed] [Google Scholar]
  17. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  18. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  19. Mott K. E., Dixon H. Collaborative study on antigens for immunodiagnosis of schistosomiasis. Bull World Health Organ. 1982;60(5):729–753. [PMC free article] [PubMed] [Google Scholar]
  20. Nash T. E. Factors that modulate clearance and ultimate fate of a specific schistosome antigen (GASP) in schistosome infections. J Immunol. 1982 Apr;128(4):1608–1613. [PubMed] [Google Scholar]
  21. Nash T. E. Localization of the circulating antigen within the gut of Schistosoma mansoni. Am J Trop Med Hyg. 1974 Nov;23(6):1085–1087. doi: 10.4269/ajtmh.1974.23.1085. [DOI] [PubMed] [Google Scholar]
  22. Nash T. Fate and mechanism of clearance of PSAP, a schistosome antigen, in schistosomiasis. J Immunol. 1983 Nov;131(5):2520–2523. [PubMed] [Google Scholar]
  23. Perlmutter R. M., Hansburg D., Briles D. E., Nicolotti R. A., Davie J. M. Subclass restriction of murine anti-carbohydrate antibodies. J Immunol. 1978 Aug;121(2):566–572. [PubMed] [Google Scholar]
  24. Qian Z. L., Deelder A. M. Schistosoma japonicum: immunological characterization and detection of circulating polysaccharide antigens from adult worms. Exp Parasitol. 1983 Apr;55(2):168–178. doi: 10.1016/0014-4894(83)90011-5. [DOI] [PubMed] [Google Scholar]
  25. Santoro F., Borojevic R., Bout D., Tachon P., Bina J. C., Capron A. Mother-child relationship in human schistosomiasis mansoni. I. Parasitic antigens and antibodies in milk. Am J Trop Med Hyg. 1977 Nov;26(6 Pt 1):1164–1168. doi: 10.4269/ajtmh.1977.26.1164. [DOI] [PubMed] [Google Scholar]
  26. Santoro F., Capron M., Joseph M., Rousseaux-Prevost R., Capron A. Circulating antigens and immune complexes in Schistosoma mansoni-infected rats. Characterization by radioimmunoprecipitation-PEG assay (RIPEGA). Clin Exp Immunol. 1978 Jun;32(3):435–442. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES