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. 2003 May 1;31(9):2279–2288. doi: 10.1093/nar/gkg347

Figure 1.

Figure 1

Fkh2p requires Mcm1p to bind the SWI5 promoter. (A) Schematic of the ternary Mcm1p–Fkh2p complex that forms on the composite CArG–Fkh binding motif in the SWI5 promoter. (B) Gel retardation analysis of in vitro translated Mcm1(1–98) and full-length Fkh2p on the CArG–fkh motif from the SWI5 promoter. The addition of Fkh2p and Mcm1p, and the locations of the DNA-bound Mcm1p and Mcm1p–Fkh2p complexes are indicated. The asterisk represents a non-specific band arising from the reticulocyte lysates.