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. 2003 May 1;31(9):2279–2288. doi: 10.1093/nar/gkg347

Figure 2.

Figure 2

Mapping the ternary complex determinants in Fkh2p. (A) A schematic of the truncated Fkh2p constructs generated for the mapping analysis. The locations of the FHA and FKH domains are indicated. The minimal region required for ternary complex formation and the location of the autoinhibitory domain are indicated. The dotted line indicates that the C-terminal end of the autoinhibitory motif is unknown. The ability of each Fkh2p construct to form ternary complexes and exhibit autoinhibitory DNA binding properties is indicated. (B and C) Gel retardation analysis of the indicated Fkh2p proteins in the presence or absence of Mcm1(1–98) (B) or in the presence of SRF(132–222) on the P(SWI5) site. Asterisks indicate Fkh2p truncations that bind DNA in the absence of Mcm1p.