Figure 4.

Fkh1p does not bind cooperatively with Mcm1p. (A) Schematic of full-length and truncated versions of Fkh1p and Fkh2p. (B) Gel retardation analysis of the indicated Fkh1p and Fkh2p truncated proteins in the absence (lanes 1–4) or presence (lanes 5–8) of Mcm1(1–98) on the P(SW15) site. The locations of complexes corresponding to Mcm1p alone, Fkh1p/Fkh2p alone or Mcm1p–Fkh2p complexes are indicated. The asterisk indicates the location of a band corresponding to a weak ternary complex.