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. 1987 Aug;69(2):291–298.

Sera of Schistosoma japonicum-infected patients cross-react with diagnostic 31/32 kD proteins of S. mansoni.

A Ruppel 1, Y E Shi 1, D X Wei 1, H J Diesfeld 1
PMCID: PMC1542415  PMID: 3115655

Abstract

Schistosoma mansoni adult worm antigens were tested for cross-reactions with sera obtained from patients infected with S. japonicum. The sera consistently recognized a doublet of bands, in immunoblots, which had molecular weights of approximately 31 and 32 kilodaltons (kD). This reaction was found to be markedly reduced with sera of patients who had received chemotherapy and who had a low risk of reinfection. Sera obtained from uninfected persons or from patients infected with other parasites never reacted with the antigen doublet. Schistosoma japonicum-infected mice produced antibodies during prepatency which predominantly recognized antigens of this molecular weight range in immunoblots performed with S. mansoni or S. japonicum proteins. Sera from S. mansoni-infected patients with a high specificity for the diagnostic S. mansoni-antigen cross-reacted with a corresponding component also in S. japonicum worms. Immunofluorescence assays performed with sera of schistosomiasis japonica patients confirmed earlier results localizing the diagnostic 31/32 kD antigens in the gut of S. mansoni. These cross-reacting 31/32 kD S. mansoni protein antigens may be applied for the immunodiagnosis of schistosomiasis japonica.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Batteiger B., Newhall W. J., 5th, Jones R. B. The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J Immunol Methods. 1982 Dec 30;55(3):297–307. doi: 10.1016/0022-1759(82)90089-8. [DOI] [PubMed] [Google Scholar]
  2. Beisler G. K., Matsuda H., Nakao M., Tanaka H. Variations of titers of immunofluorescent antibody against cercaria, adult and egg antigens developed in rabbits infected with Schistosoma japonicum before and after treatment with praziquantel. Jpn J Exp Med. 1984 Feb;54(1):17–22. [PubMed] [Google Scholar]
  3. Carlier Y., Bout D., Strecker G., Debray H., Capron A. Purification, immunochemical, and biologic characterization of the Schistosoma circulating M antigen. J Immunol. 1980 May;124(5):2442–2450. [PubMed] [Google Scholar]
  4. Chappell C. L., Dresden M. H. Schistosoma mansoni: proteinase activity of "hemoglobinase" from the digestive tract of adult worms. Exp Parasitol. 1986 Apr;61(2):160–167. doi: 10.1016/0014-4894(86)90148-7. [DOI] [PubMed] [Google Scholar]
  5. Cruise K. M., Mitchell G. F., Garcia E. G., Tiu W. U., Hocking R. E., Anders R. F. Sj23, the target antigen in Schistosoma japonicum adult worms of an immunodiagnostic hybridoma antibody. Parasite Immunol. 1983 Jan;5(1):37–45. doi: 10.1111/j.1365-3024.1983.tb00721.x. [DOI] [PubMed] [Google Scholar]
  6. Deelder A. M., Kornelis D. Immunodiagnosis of recently acquired Schistosoma mansoni infection. A comparison of various immunological techniques. Trop Geogr Med. 1981 Mar;33(1):36–41. [PubMed] [Google Scholar]
  7. Deelder A. M., Reinders P. N., Rotmans J. P. Purification studies on an acidic protease from adult Schistosoma mansoni. Acta Leiden. 1977 Dec;45:91–103. [PubMed] [Google Scholar]
  8. Hillyer G. V., Nieves-Frau L. L., Vazquez G. Identification of a genus-specific Schistosoma mansoni soluble egg antigen reactive with the serum of infected patients. Am J Trop Med Hyg. 1986 Nov;35(6):1198–1204. doi: 10.4269/ajtmh.1986.35.1198. [DOI] [PubMed] [Google Scholar]
  9. Kasschau M. R., Robinson D. C., Dreden M. H. Schistosoma mansoni: activation of hemolytic activity in homogenates from live adult worms. Exp Parasitol. 1986 Dec;62(3):442–449. doi: 10.1016/0014-4894(86)90053-6. [DOI] [PubMed] [Google Scholar]
  10. Kelsoe G. H., Weller T. H. Immunodiagnosis of infection with Schistosoma mansoni: enzyme-linked immunosorbent assay for detection of antibody to circulating antigen. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5715–5717. doi: 10.1073/pnas.75.11.5715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lewert R. M. A brief review of seroepidemiologic and quantitative stool techniques relative to schistosomiasis japonica. Southeast Asian J Trop Med Public Health. 1984 Dec;15(4):482–485. [PubMed] [Google Scholar]
  12. Lindquist R. N., Senft A. W., Petitt M., McKerrow J. Schistosoma mansoni: purification and characterization of the major acidic proteinase from adult worms. Exp Parasitol. 1986 Jun;61(3):398–404. doi: 10.1016/0014-4894(86)90196-7. [DOI] [PubMed] [Google Scholar]
  13. Mitchell G. F., Beall J. A., Cruise K. M., Tiu W. U., Garcia E. G. Antibody responses to the antigen Sj26 of Schistosoma japonicum worms that is recognized by genetically resistant 129/J mice. Parasite Immunol. 1985 Mar;7(2):165–178. doi: 10.1111/j.1365-3024.1985.tb00067.x. [DOI] [PubMed] [Google Scholar]
  14. Mott K. E. Diagnostic tools for Schistosoma japonicum in control programmes. Arzneimittelforschung. 1984;34(9B):1217–1220. [PubMed] [Google Scholar]
  15. Mott K. E., Dixon H. Collaborative study on antigens for immunodiagnosis of schistosomiasis. Bull World Health Organ. 1982;60(5):729–753. [PMC free article] [PubMed] [Google Scholar]
  16. Nash T. E., Deelder A. M. Comparison of four schistosome excretory-secretory antigens: phenol sulfuric test active peak, cathodic circulating antigen, gut-associated proteoglycan, and circulating anodic antigen. Am J Trop Med Hyg. 1985 Mar;34(2):236–241. doi: 10.4269/ajtmh.1985.34.236. [DOI] [PubMed] [Google Scholar]
  17. Nash T. E., Lunde M. N., Cheever A. W. Analysis and antigenic activity of a carbohydrate fraction derived from adult Schistosoma mansoni. J Immunol. 1981 Feb;126(2):805–810. [PubMed] [Google Scholar]
  18. Norden A. P., Strand M. Schistosoma mansoni, S. haematobium, and S. japonicum: identification of genus- and species-specific antigenic egg glycoproteins. Exp Parasitol. 1984 Dec;58(3):333–344. doi: 10.1016/0014-4894(84)90050-x. [DOI] [PubMed] [Google Scholar]
  19. Norden A. P., Strand M. Schistosoma mansoni, S. haematobium, and S. japonicum: identification of genus-, species-, and gender-specific antigenic worm glycoproteins. Exp Parasitol. 1984 Feb;57(1):110–123. doi: 10.1016/0014-4894(84)90070-5. [DOI] [PubMed] [Google Scholar]
  20. Ruppel A., Diesfeld H. J., Rother U. Immunoblot analysis of Schistosoma mansoni antigens with sera of schistosomiasis patients: diagnostic potential of an adult schistosome polypeptide. Clin Exp Immunol. 1985 Dec;62(3):499–506. [PMC free article] [PubMed] [Google Scholar]
  21. Ruppel A., Rother U., Vongerichten H., Lucius R., Diesfeld H. J. Schistosoma mansoni: immunoblot analysis of adult worm proteins. Exp Parasitol. 1985 Oct;60(2):195–206. doi: 10.1016/0014-4894(85)90023-2. [DOI] [PubMed] [Google Scholar]
  22. Sauer M. C., Senft A. W. Properties of a proteolytic enzyme from Schistosoma mansoni. Comp Biochem Physiol B. 1972 Jun 15;42(2):205–220. doi: 10.1016/0305-0491(72)90267-2. [DOI] [PubMed] [Google Scholar]
  23. Smith D. B., Davern K. M., Board P. G., Tiu W. U., Garcia E. G., Mitchell G. F. Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8703–8707. doi: 10.1073/pnas.83.22.8703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. TIMMS A. R., BUEDING E. Studies of a proteolytic enzyme from Schistosoma mansoni. Br J Pharmacol Chemother. 1959 Mar;14(1):68–73. doi: 10.1111/j.1476-5381.1959.tb00930.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tracy J. W., Domingo E. O., Mahmoud A. A. Evaluation of a purified Schistosoma japonicum glycoprotein egg antigen for the immunodiagnosis of infection in man. Am J Trop Med Hyg. 1985 Jan;34(1):92–95. doi: 10.4269/ajtmh.1985.34.92. [DOI] [PubMed] [Google Scholar]
  26. Tsang V. C., Hancock K., Maddison S. E., Beatty A. L., Moss D. M. Demonstration of species-specific and cross-reactive components of the adult microsomal antigens from Schistosoma mansoni and S. japonicum (MAMA and JAMA). J Immunol. 1984 May;132(5):2607–2613. [PubMed] [Google Scholar]
  27. Tsang V. C., Tsang K. R., Hancock K., Kelly M. A., Wilson B. C., Maddison S. E. Schistosoma mansoni adult microsomal antigens, a serologic reagent. I. Systematic fractionation, quantitation, and characterization of antigenic components. J Immunol. 1983 Mar;130(3):1359–1365. [PubMed] [Google Scholar]
  28. Watt G., Long G. W., Ranoa C. P., Cross J. H. The diagnosis of Schistosoma japonicum infection. Areas of uncertainty. Trans R Soc Trop Med Hyg. 1986;80(1):10–11. doi: 10.1016/0035-9203(86)90184-7. [DOI] [PubMed] [Google Scholar]
  29. Yogore M. G., Jr, Lewert R. M., Blas B. L. Seroepidemiology of schistosomiasis japonica by ELISA in the Philippines. I. Underestimation by stool examination of the prevalence of infection in school children. Am J Trop Med Hyg. 1983 Nov;32(6):1322–1334. doi: 10.4269/ajtmh.1983.32.1322. [DOI] [PubMed] [Google Scholar]
  30. van Helden H. P., Terpstra W. J., Okot-Kotber B. M., Eyakuze V. M. Are there stage-characteristic immunofluorescence patterns in schistosomiasis? Trans R Soc Trop Med Hyg. 1975;69(3):309–311. doi: 10.1016/0035-9203(75)90124-8. [DOI] [PubMed] [Google Scholar]

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