FIG. 3.
PE induces p300 expression, acetylation, and DNA binding of GATA-4 in cardiac myocytes. (A) Primary cardiac myocytes from neonatal rats were stimulated with saline (SS) or PE (10−5 M) for 48 h. Nuclear extracts from these cells were subjected to Western blotting with anti-GATA-4 antibody, anti-p300 antibody, anti-PCAF antibody, or anti-β-actin antibody. (B) The same nuclear extracts (100 μg of protein) were immunoprecipitated with anti-GATA-4 antibody and sequentially subjected to Western blotting with anti-acetylated lysine antibody and with anti-GATA-4 antibody. (C and D) The same nuclear extracts were probed with a radiolabeled double-stranded oligonucleotide containing the ET-1 GATA site (C) and with one containing the Sp-1 site (D). −, absent.