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. 2003 May;23(10):3593–3606. doi: 10.1128/MCB.23.10.3593-3606.2003

FIG. 7.

FIG. 7.

Cardiac overexpression of p300 results in acetylation and increased DNA-binding activity of GATA-4. (A) Nuclear extracts (10 μg of protein) from WT or p300 TG mouse hearts were subjected to Western blotting with anti-p300 antibody, anti-GATA-4 antibody, or anti-β-actin antibody. (B) Nuclear extracts (400 μg of protein) from WT or TG mouse hearts were immunoprecipitated with anti-GATA-4 antibody and control goat IgG and sequentially subjected to Western blotting with anti-acetylated lysine antibody and anti-GATA-4 antibody. (C and D) Nuclear extracts from WT and TG mouse hearts were probed with a radiolabeled double-stranded oligonucleotide containing the GATA-4 site in the ET-1 promoter (C) and with one containing the Sp-1 site (D). Small arrow, supershifted band of GATA-4. (E) Analysis of ET-1 mRNA levels in WT and TG mouse hearts was performed by RT-PCR. (F) Northern blotting of total RNA from WT and TG mouse hearts for β-MHC, ANF, and GAPDH.