Skip to main content
. 2003 May;23(10):3427–3441. doi: 10.1128/MCB.23.10.3427-3441.2003

FIG. 1.

FIG. 1.

Fli-1 interacts with GATA-1. (A) Yeast strain AH109 was transformed with the indicated plasmids as shown and plated onto the corresponding sectors shown in panels B and C. (B and C) Growth of AH109 yeast harboring the plasmids as shown in panel A after 5 days of incubation at 30°C on minimal medium lacking Leu and Trp (B) and minimal medium lacking Ade, His, Leu, and Trp (C). (D) Coimmunoprecipitation of Fli-1 with GATA-1. HeLa cells were transfected with cDNAs encoding for pcDNA3 backbone (lane 1), GATA-1 (lane 2), Fli-1 and GATA-1 (lane 3), or Fli-1 alone (lanes 4 and 5) and were immunoprecipitated with anti-GATA-1 monoclonal antibody (lanes 1 to 4) or anti-Fli-1 monoclonal antibody as control (lane 5). Immunoprecipitates were then subjected to immunoblotting with anti-GATA-1 or anti-Fli-1 monoclonal antibody, as outlined in Materials and Methods.