FIG. 3.

GATA domains required for the Fli-1-GATA-1 interaction. (A) Schematic representations of the GST-GATA-1 constructs used in GST pulldown assays. (B) GST pulldown assays using the GST-GATA-1 deletions and mutations shown schematically above. Lane 1, 10% of the input in vitro-translated ³⁵S-labeled Fli-1 protein; lane 2, GST-coated beads; lanes 3 to 5, GST-GATA constructs, as indicated. (C) Finer mapping of the domains of GATA-1 required for the interaction with Fli-1. Lane 1, 33% of the input in vitro-translated ³⁵S-labeled Fli-1 protein; lane 2, GST; lane 3, GATA-C finger with a mutated cysteine C258A; lane 4, wild-type C finger; lanes 5 to 9, GATA-N finger constructs, as shown. Samples were incubated as described in the legend for Fig. 2.