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. 2003 May;23(10):3427–3441. doi: 10.1128/MCB.23.10.3427-3441.2003

FIG. 8.

FIG. 8.

Fli-1 and GATA-1 exhibit cooperative DNA binding. (A) EMSA of equilibrium binding studies of increasing amounts of MBP-GATA-NC titrated onto the 32P-labeled GPIX-GATA-Ets oligonucleotide (0.2 fmol/20-μl reaction). The wedge indicates increasing concentrations of MBP-GATA-NC (2 × 10−13 M to 2 × 10−8 M). G.D corresponds to GATA-1-DNA binary complex. DD corresponds to GATA-1 dimer. (B) EMSA, as described for panel A, with increasing amounts of GST-Fli-1 Ets (5 × 10−13 M to 5 × 10−7 M). F.D corresponds to Fli-1-DNA binary complex. (C) EMSA, as described for panel A, with identical amounts of MBP-GATA-NC and probe in the presence of a saturating amount of GST-Fli-1. F.D corresponds to Fli-1-DNA complex, G.D corresponds to GATA-1-DNA complex, and G.F.D corresponds to GATA-1-Fli-1-DNA ternary complex.