Figure 5.

Transcripts of Chlase gene AtCLH2 is present in coi1 flowers. A, Northern-blot analyses of the same RNA samples shown in Figure 4B probed with the AtCLH2 cDNA. Transcripts of AtCLH2 are detected at low levels in wild-type (wt), coi1 mutant, transgenic sense (wtS, coi1S), and antisense (wtA) plants (arrowhead). B, Southern-blot analysis of AtCLH2 expression by reverse transcriptase (RT)-PCR. The amplified fragments were blotted, transferred to a nylon membrane, and hybridized to the AtCLH2 cDNA. AtCLH2 transcripts were detected in leaves (L), flowers (F), and flower buds (B) of both wild type and coi1 mutant. The gene was not detected in roots (R) and MeJA did not induce it in leaves (M).