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. 2002 Apr;128(4):1264–1270. doi: 10.1104/pp.010785

Figure 1.

Figure 1

Expression of RAmy1A (A), OsGA3ox1, and OsGA3ox2 (B) in germinating rice seeds. A (a), Northern analysis using the RAmy1A probe. Seeds were germinated for the indicated hours after imbibition with or without uniconazol (uni. + or −), 5 μg of total RNA was prepared from the treated and untreated embryo, and 1 μg of total RNA was prepared from the treated and untreated endosperm. A (b), Relative amount of RAmy1A transcript in the embryo (diamonds) and endosperm (circles) in the presence (black symbols) or absence (white symbols) of uniconazol. The level of the RAmy1A transcript was normalized against the level of the actin transcript. The normalized values shown here are relative to the expression levels detected in endosperm of untreated seeds at 72 h (100%). B (a), Northern analysis of OsGA3ox2 and OsGA3ox1 in embryo. Five micrograms of total RNA was prepared to detect OsGA3ox2, and 20 μg of total RNA was prepared to detect OsGA3ox1. B (b), Relative level of the OsGA3ox1 (triangles) and OsGA3ox2 (squares) transcripts in the germinating rice embryo treated with (black symbols) or without (white symbols) uniconazol. Normalization of OsGA3ox expression is the same as in A.