Figure 1.

Induction of PCD by oxidative stress. A, Cells were incubated with 10 mm G and 2, 5, or 10 units mL⁻¹ GO. Cell death was measured 1, 3, 6, and 18 h later. Each point represents mean value of three independent experiments. B, Inhibition of oxidative stress-induced death by protease inhibitors. Arabidopsis cells were treated with 10 mm G plus 10 units mL⁻¹ GO as in A in the presence of l-trans-epoxysuccinyl-leucylamido(4-guanidino) butane (50 μm), AEBSF (1 mm), or caspase-3 (Casp3) inhibitor (50 μm), and cell death was measured after 18 h. Results are mean values of three independent replica.