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Purification of specific [14C]spd-binding activity from maize coleoptiles (260 g fresh wt)
| Purification Step | Protein | Specific [14C]spd Binding | Specific Activity | Recovery |
|---|---|---|---|---|
| mg | pmol | pmol mg protein−1 | % | |
| Acetone powders (crude extract) | 120 | 475 | 4.0 | 100 |
| DEAE (0.2 m NaCl) | 83.2 | 71.6 | 0.9 | 15.0 |
| Octyl-agarose (buffer fraction) | 3.4 | 64.2 | 18.9 | 13.5 |
| HiTrapQ (fraction 5) | 0.005 | 32.6 | 6270 | 6.9 |
The binding activity was measured by glass fiber filter assay. Values are the mean of eight different experiments.
