Figure 5.

RNA expression of the Arabidopsis ERF genes in response to pathogen infection and SA treatment in the pad4-1 mutant. A, Infection of WT and pad4-1 with P. syringae. Arabidopsis leaves from 4-week-old plants were infiltrated with a mock solution (m) or with the same solution containing approximately 10⁷cfu mL⁻¹ of the avirulent pathogen Pst DC3000(avrRpt2) (av) or approximately 10⁷cfu mL⁻¹ of the virulent pathogen Pst DC3000 (vir). RNAs isolated from leaves at the indicated time points were subjected to RT-PCR. B, Treatment of WT and pad4-1 with SA. Four-week-old Arabidopsis plants were sprayed with 0.1% (v/v) ethanol (−) or 1 mm SA or were not treated (nt). RNAs isolated from leaves after 6 h of treatment were analyzed by RT-PCR.