Table I.
Construct | Line | Expression Level of DmAMP1 | Expression Level of RsAFP2 |
---|---|---|---|
% | |||
pFAJ3105 | 1 | 0.77 | 0.29 |
2 | 1.13 | 0.22 | |
3 | 0.48 | 0.20 | |
4 | 0.005 | <0.001 | |
5 | 0.36 | 0.05 | |
6 | 0.99 | 0.25 | |
7 | 0.60 | 0.09 | |
8 | 0.13 | <0.001 | |
9 | 0.25 | 0.08 | |
10 | 1.35 | 0.35 | |
11 | 0.24 | 0.07 | |
12 | 1.18 | 0.24 | |
13 | 0.68 | 0.17 | |
14 | 0.49 | 0.07 | |
Average | 0.62 | 0.15 | |
pFAJ3109 | 1 | 0.19 | nda |
2 | 0.05 | nd | |
3 | 0.02 | nd | |
4 | 0.20 | nd | |
5 | 0.10 | nd | |
6 | 0.06 | nd | |
7 | 0.07 | nd | |
8 | 0.003 | nd | |
Average | 0.09 | nd |
Expression levels of DmAMP1 and RsAFP2 are expressed as the ratio of the amount of DmAMP1-CRP or RsAFP2-CRP to the amount of total soluble protein in crude extracts from leaves of transgenic Arabidopsis plants. The Arabidopsis plants are transformed with either the double protein construct (pFAJ3105) coding for DmAMP1 and RsAFP2 or the single protein construct (pFAJ3109) coding only for DmAMP1.
nd, Not determined.