Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1987 Jun;68(3):528–534.

A common anti-DNA idiotype and other autoantibodies in sera of offspring of mothers with systemic lupus erythematosus.

A el-Roeiy 1, N Gleicher 1, D Isenberg 1, R C Kennedy 1, Y Shoenfeld 1
PMCID: PMC1542743  PMID: 3115649

Abstract

Since the immune response in fetuses of mothers with systemic lupus erythematosus (SLE) is unknown, we investigated sera from six mothers and their paired offspring by enzyme-linked immunosorbent assay (ELISA) for the presence of a common anti-DNA idiotype (16/6 Id) and, as control, for the presence of an unrelated public idiotype of antibody to hepatitis B surface antigen (HBsAg). In addition, maternal as well as fetal sera were evaluated for the presence of antibodies to ssDNA, dsDNA, poly(I), poly (dT), RNA, cardiolipin, total histones and the presence of lupus anticoagulant. Clinically active SLE mothers showed in general increased IgG and, to a lesser extent, IgM autoantibody activity. Circulating lupus anticoagulant was detectable in clinically active mothers only. All offspring of clinically active SLE mothers showed increased IgG autoantibodies to a variety of antigens, while IgM antibodies were detected in only one fetus. In contrast, fetuses of clinically inactive mothers showed only minor IgG activity. Common anti-DNA-idiotype (16/6 Id) activity also correlated with disease activity in both maternal and fetal compartments. One clinically active mother was 16/6-negative; her offspring was, however, positive, indicating de novo production of the idiotype by the fetus. In contrast, a control anti-HBsAg idiotype was not detected in either maternal or fetal sera. It therefore appears that offspring of clinically active SLE mothers serologically reflect maternal disease activity. Furthermore, autoantibodies and common idiotype of autoantibodies can be found within the fetal compartment even in the absence of such antibodies in the maternal serum. Discrepancies between mothers and offspring in IgM-autoantibody levels and the presence of new idiotypes in fetuses are indicative of fetal de novo autoantibody production.

Full text

PDF
531

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abramowsky C. R., Vegas M. E., Swinehart G., Gyves M. T. Decidual vasculopathy of the placenta in lupus erythematosus. N Engl J Med. 1980 Sep 18;303(12):668–672. doi: 10.1056/NEJM198009183031204. [DOI] [PubMed] [Google Scholar]
  2. El-Roeiy A., Shoenfeld Y. Autoimmunity and pregnancy. Am J Reprod Immunol Microbiol. 1985 Sep;9(1):25–32. doi: 10.1111/j.1600-0897.1985.tb00337.x. [DOI] [PubMed] [Google Scholar]
  3. Gimovsky M. L., Montoro M., Paul R. H. Pregnancy outcome in women with systemic lupus erythematosus. Obstet Gynecol. 1984 May;63(5):686–692. [PubMed] [Google Scholar]
  4. Isenberg D. A., Shoenfeld Y., Madaio M. P., Rauch J., Reichlin M., Stollar B. D., Schwartz R. S. Anti-DNA antibody idiotypes in systemic lupus erythematosus. Lancet. 1984 Aug 25;2(8400):417–422. doi: 10.1016/s0140-6736(84)92904-0. [DOI] [PubMed] [Google Scholar]
  5. Isenberg D. A., Shoenfeld Y., Schwartz R. S. Multiple serologic reactions and their relationship to clinical activity in systemic lupus erythematosus. Arthritis Rheum. 1984 Feb;27(2):132–138. doi: 10.1002/art.1780270203. [DOI] [PubMed] [Google Scholar]
  6. Isenberg D. A., Shoenfeld Y., Walport M., Mackworth-Young C., Dudeney C., Todd-Pokropek A., Brill S., Weinberger A., Pinkas J. Detection of cross-reactive anti-DNA antibody idiotypes in the serum of systemic lupus erythematosus patients and of their relatives. Arthritis Rheum. 1985 Sep;28(9):999–1007. doi: 10.1002/art.1780280907. [DOI] [PubMed] [Google Scholar]
  7. Kennedy R. C., Dreesman G. R. Common idiotypic determinant associated with human antibodies to hepatitis B surface antigen. J Immunol. 1983 Jan;130(1):385–389. [PubMed] [Google Scholar]
  8. Kennedy R. C., Dreesman G. R. Enhancement of the immune response to hepatitis B surface antigen. In vivo administration of antiidiotype induces anti-HBs that expresses a similar idiotype. J Exp Med. 1984 Mar 1;159(3):655–665. doi: 10.1084/jem.159.3.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee L. A., Bias W. B., Arnett F. C., Jr, Huff J. C., Norris D. A., Harmon C., Provost T. T., Weston W. L. Immunogenetics of the neonatal lupus syndrome. Ann Intern Med. 1983 Nov;99(5):592–596. doi: 10.7326/0003-4819-99-5-592. [DOI] [PubMed] [Google Scholar]
  10. Morrow W. J., Williams D. J., Ferec C., Casburn-Budd R., Isenberg D. A., Paice E., Snaith M. L., Youinou P., Le Goff P. The use of C3d as a means of monitoring clinical activity in systemic lupus erythematosus and rheumatoid arthritis. Ann Rheum Dis. 1983 Dec;42(6):668–671. doi: 10.1136/ard.42.6.668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nakao K., Nishitani H., Suzuki M., Ohta M., Hayashi K. Anti-acetylcholine receptor IgG in neonatal myasthenia gravis. N Engl J Med. 1977 Jul 21;297(3):169–170. doi: 10.1056/NEJM197707212970315. [DOI] [PubMed] [Google Scholar]
  12. Papalian M., Lafer E., Wong R., Stollar B. D. Reaction of systemic lupus erythematosus antinative DNA antibodies with native DNA fragments from 20 to 1,200 base pairs. J Clin Invest. 1980 Feb;65(2):469–477. doi: 10.1172/JCI109690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schlamowitz M. Membrane receptors in the specific transfer of immunoglobulins from mother to young. Immunol Commun. 1976;5(6):481–500. doi: 10.3109/08820137609033862. [DOI] [PubMed] [Google Scholar]
  14. Schleider M. A., Nachman R. L., Jaffe E. A., Coleman M. A clinical study of the lupus anticoagulant. Blood. 1976 Oct;48(4):499–509. [PubMed] [Google Scholar]
  15. Scott J. S., Maddison P. J., Taylor P. V., Esscher E., Scott O., Skinner R. P. Connective-tissue disease, antibodies to ribonucleoprotein, and congenital heart block. N Engl J Med. 1983 Jul 28;309(4):209–212. doi: 10.1056/NEJM198307283090403. [DOI] [PubMed] [Google Scholar]
  16. Shoenfeld Y., Hsu-Lin S. C., Gabriels J. E., Silberstein L. E., Furie B. C., Furie B., Stollar B. D., Schwartz R. S. Production of autoantibodies by human-human hybridomas. J Clin Invest. 1982 Jul;70(1):205–208. doi: 10.1172/JCI110595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shoenfeld Y., Isenberg D. A., Rauch J., Madaio M. P., Stollar B. D., Schwartz R. S. Idiotypic cross-reactions of monoclonal human lupus autoantibodies. J Exp Med. 1983 Sep 1;158(3):718–730. doi: 10.1084/jem.158.3.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shoenfeld Y., Rauch J., Massicotte H., Datta S. K., André-Schwartz J., Stollar B. D., Schwartz R. S. Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas. N Engl J Med. 1983 Feb 24;308(8):414–420. doi: 10.1056/NEJM198302243080802. [DOI] [PubMed] [Google Scholar]
  19. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  20. Vanderbeeken Y., Sarfati M., Bose R., Delespesse G. In utero immunization of the fetus to tetanus by maternal vaccination during pregnancy. Am J Reprod Immunol Microbiol. 1985 Jun;8(2):39–42. doi: 10.1111/j.1600-0897.1985.tb00347.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES