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Purification of the Synechocystis SPP expressed in E. coli. Proteins were separated by SDS-PAGE in a 12% (w/v) gel and visualized by staining with Coomassie Blue R250. Lane A, 10-kD ladder protein molecular mass markers. Lane B, Total soluble extract from E. coli (pTYB2/Synspp) noninduced (20 μg). Lane C, Total soluble extract from E. coli (pTYB2/Synspp) IPTG-induced (10 μg). Lane D, 29% (w/v) PEG, pH 5 precipitate (2 μg). Lane E, Superdex 200 fraction (2 μg).
