Table 1.
Polymorphic nucleotides in the “marker” intron used to identify cadherin alleles
| Marker | 31 | 92 | 143 | 183 | 196 | 200 | 221–224 | 284 | 380 | 381 | 383 | 408 | 419 | 429 | 434 | 440 | 447 | 461–463 | 488 | 513 | 520 | 530 | 538 | 540 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| mr1 | A | A | C | G | T | A | … | G | C | A | T | C | T | A | T | C | A | … | G | G | C | C | T | G |
| mr2 | C | G | C | G | G | A | … | G | A | G | T | C | C | C | C | T | T | AAC | A | A | T | T | G | C |
| mr3 | C | G | A | A | G | G | … | A | C | G | G | T | C | C | C | C | T | AAC | A | A | C | C | T | G |
| ms1 | C | G | C | A | G | G | TAAG | G | C | G | T | C | C | C | C | C | T | AAC | A | A | C | C | T | G |
| ms2 | A | A | C | G | T | A | … | G | C | A | T | C | T | A | T | C | A | … | G | G | C | C | T | G |
This intron occurs between nucleotides encoding amino acids 1143 and 1144 (Fig. 2). The first 540 bp of the intron were amplified. PCR products were recovered from agarose gels using the QIAquick gel extraction kit (Qiagen) and sequenced. Diagnostic nucleotides are shown in boldface. Dots indicate deletions.