Figure 2.

Specific Gβγ combinations are not required for coupling between α₂-ARs and N-type Ca²⁺ channels. (A) Superimposed current traces in the absence or presence of 10 μM NE recorded from neurons expressing Gα_oA(C351G) and different Gβγ combinations (β₁γ₂, β₁γ₃, β₂γ₂, β₃γ₄, β₄γ₄, β₅γ₂). (B) Summary of VD Ca²⁺ current inhibition by NE under conditions described above. Note that tonic inhibition is apparent in neurons expressing Gα_oA(C351G) β₅γ₂. Data are presented as mean ± SEM, and numbers in parentheses indicate the number of neurons tested.